9QND
Connexin-32 (Cx32) W3S mutant gap junction channel in POPC-containing MSP2N2 nanodiscs
EM specimen
| Vitrification | Yes |
EM Data Collection
| Microscope | TFS KRIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Detector | GATAN K3 BIOQUANTUM (6k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 2.35 |
| Software | RELION,PHENIX,CTFFIND |
| Symmetry | D6 |
| CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |
