9QLT

Crystal Structure of Dimeric Apo-D154A-FNR of A. Fischeri

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID30B
Synchrotron site	ESRF
Beamline	ID30B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-03-30
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9677
Spacegroup name	I 4 2 2
Unit cell lengths	74.850, 74.850, 208.630
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	36.450 - 2.400
R-factor	0.2145
Rwork	0.214
R-free	0.22560
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.187
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.21.2_5419)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.200	2.480
High resolution limit [Å]	2.400	2.400
Rmerge	0.092	2.705
Number of reflections	12049	1156
<I/σ(I)>	15.5
Completeness [%]	99.6
Redundancy	11.1
CC(1/2)	0.999	0.482

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	MPD, HEPES, ANAEROBIC