9QLR

Nonamer crystal structure of the transcription factor MraZ from Mycoplasma genitalium

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-10-01
Detector	DECTRIS PILATUS 200K
Wavelength(s)	0.77490
Spacegroup name	P 1 21 1
Unit cell lengths	125.621, 203.821, 149.301
Unit cell angles	90.00, 111.76, 90.00

Refinement procedure

Resolution	82.120 - 3.850
R-factor	0.2513
Rwork	0.248
R-free	0.30770
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.780
Data reduction software	autoPROC
Data scaling software	autoPROC
Phasing software	PHASER
Refinement software	PHENIX ((1.17.1_3660: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	82.120	4.290
High resolution limit [Å]	3.850	3.850
Number of reflections	31414	1572
<I/σ(I)>	3.4
Completeness [%]	83.0
Redundancy	3.3
CC(1/2)	0.994	0.663

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		291	12.00 % w/v Polyethylene glycol 6,000, 2.00 M Sodium chloride