9QLG

Crystal structure of the transcription factor MraZ from Mycoplasma genitalium

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALBA BEAMLINE XALOC
Synchrotron site	ALBA
Beamline	XALOC
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-11-19
Detector	DECTRIS PILATUS 300K
Wavelength(s)	0.97926
Spacegroup name	I 4
Unit cell lengths	143.100, 143.100, 71.460
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.250 - 1.960
R-factor	0.1909
Rwork	0.188
R-free	0.24460
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.855
Data reduction software	autoPROC
Data scaling software	autoPROC
Phasing software	PHASER
Refinement software	PHENIX ((1.17.1_3660: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	101.187	2.119
High resolution limit [Å]	1.956	1.956
Number of reflections	37663	1883
<I/σ(I)>	3.9
Completeness [%]	87.3
Redundancy	3.8
CC(1/2)	0.774	0.116

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	2.0 M Ammonium sulfate 0.1 M Sodium cacodylate pH 6.5, 0.2 M Sodium chloride