9QLF

Human myoferlin (1-1997) in complex with an MSP2N2 lipid nanodisc (25 mol% DOPS, 5 mol% PI(4,5)P2 and 5 mol% Cholesterol)

This is a non-PDB format compatible entry.

EM specimen

Vitrification	Yes
Instrument	FEI VITROBOT MARK IV

EM Data Collection

Microscope	TFS KRIOS
Electron Gun	FIELD EMISSION GUN
Accelerating Voltage (kV)	300
Cs (mm)	2.7
Energy Filter	TFS Selectris
Detector	FEI FALCON IV (4k x 4k)

3D Reconstruction

Method	SINGLE PARTICLE
Resolution (Å)	2.65
Number of Particle	5039421
Software	cryoSPARC,PHENIX,EPU,ISOLDE,UCSF ChimeraX
Symmetry	C1
CTF correction	PHASE FLIPPING AND AMPLITUDE CORRECTION