9QGZ

Crystal structure of a D-lactate dehydrogenase from Porcellio dilatatus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID30B
Synchrotron site	ESRF
Beamline	ID30B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-05-15
Detector	DECTRIS EIGER2 X 9M
Wavelength(s)	0.873128
Spacegroup name	P 1
Unit cell lengths	69.668, 103.920, 143.827
Unit cell angles	81.94, 77.24, 75.41

Refinement procedure

Resolution	78.150 - 2.840
R-factor	0.2101
Rwork	0.207
R-free	0.26230
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.887
Data reduction software	autoPROC
Data scaling software	autoPROC
Phasing software	PHASER
Refinement software	PHENIX ((1.19.2_4158: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	78.150	3.230
High resolution limit [Å]	2.840	2.840
Rpim	0.103
Number of reflections	46387	2319
<I/σ(I)>	3.7
Completeness [%]	89.2
Redundancy	1.8
CC(1/2)	0.987	0.571

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293.15	0.2 M sodium formate, 0.1 M sodium phosphate pH 6.2, 20 % (v/v) PEG Smear Medium and 10 % (v/v) glycerol