Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-04-21 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97950 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 41.458, 84.691, 176.383 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 88.190 - 1.879 |
| R-factor | 0.2795 |
| Rwork | 0.278 |
| R-free | 0.30430 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.900 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | BUSTER (2.11.8 (24-FEB-2021)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 88.192 | 2.072 |
| High resolution limit [Å] | 1.879 | 1.879 |
| Rmerge | 0.053 | 0.716 |
| Rmeas | 0.056 | 0.752 |
| Number of reflections | 33881 | 1694 |
| <I/σ(I)> | 24 | |
| Completeness [%] | 89.1 | |
| Redundancy | 10 | |
| CC(1/2) | 1.000 | 0.953 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 10-20% (w/v) PEG3350, 5% (v/v) ethanol and 100mM PCTP pH 8.0 |
