9QD1

Structure of J paramyxovirus virus receptor binding protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04-1
Synchrotron site	Diamond
Beamline	I04-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-02-03
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.9282
Spacegroup name	P 61
Unit cell lengths	164.240, 164.240, 112.470
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	46.400 - 2.200
R-factor	0.1903
Rwork	0.189
R-free	0.20710
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.632
Data reduction software	xia2
Data scaling software	xia2
Phasing software	PHASER
Refinement software	PHENIX ((1.21.1_5286: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	60.110	2.240
High resolution limit [Å]	2.200	2.200
Rmerge	0.180	2.340
Rmeas	0.190	2.460
Rpim	0.060	0.760
Number of reflections	87311	2889
<I/σ(I)>	9.6	1.3
Completeness [%]	100.0
Redundancy	10.2
CC(1/2)	0.990	0.480

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	293	Crystals grew in 0.1 M carboxylic acid, 0.1 M tris/bicine pH 8.5, 6% sucrose, 0.2 M ammonium sulphate, 37.5% Morpheus (Molecular dimensions) precipitant mix 4, consisting of 25% v/v 2-methyl-2,4-pentanediol (MPD), 25% w/v PEG 1000 (P1k) and 25% w/v polyethylene glycol 3350 (PEG 3350).