9QCU
Crystal structure of Rhizobium etli L-asparaginase ReAV S48A mutant
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1) |
Synchrotron site | PETRA III, EMBL c/o DESY |
Beamline | P13 (MX1) |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2022-07-08 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9150 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 78.041, 91.340, 105.871 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 51.760 - 1.400 |
R-factor | 0.1492 |
Rwork | 0.149 |
R-free | 0.17870 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.010 |
RMSD bond angle | 1.183 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 78.040 | 1.490 |
High resolution limit [Å] | 1.400 | 1.400 |
Rmerge | 0.084 | 1.783 |
Rmeas | 0.087 | 1.857 |
Number of reflections | 148671 | 23779 |
<I/σ(I)> | 17.97 | 1.48 |
Completeness [%] | 100.0 | |
Redundancy | 13.3 | |
CC(1/2) | 1.000 | 0.671 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 292 | 30% PEG 4000, 0.2 M Li2SO4, 0.1 M Tris pH 8.5, 0.2 M Glycine |