9QCA

PRMT2 in complex with MMA analog

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-06-15
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.979
Spacegroup name	C 2 2 21
Unit cell lengths	66.405, 114.994, 133.226
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.720 - 1.730
R-factor	0.1658
Rwork	0.165
R-free	0.18540
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.156
Data reduction software	HKL-2000 (0.7.8)
Data scaling software	Aimless (2.3.15)
Phasing software	PHENIX (1.20.1_4487)
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.780	1.740
High resolution limit [Å]	1.710	1.710
Rmerge	0.105	0.449
Rmeas	0.110	0.475
Rpim	0.031	0.152
Number of reflections	53528	9359
<I/σ(I)>	21	2.8
Completeness [%]	96.5	37
Redundancy	12.6	8.8
CC(1/2)	0.998	0.914

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	293	20% peg3350, 100 mM hepes pH7, 0.1M MgSO4