9QB7

Structure of rat Phospholipase C gamma 1 mutant S345F

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-12-13
Detector	DECTRIS EIGER2 XE 16M
Wavelength(s)	0.953738
Spacegroup name	P 21 21 21
Unit cell lengths	72.442, 82.391, 230.676
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.200 - 2.560
R-factor	0.1904
Rwork	0.188
R-free	0.23330
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.045
Data reduction software	autoPROC (1.0.5)
Data scaling software	autoPROC (1.0.5)
Refinement software	PHENIX (1.21.1_5286)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	115.338	2.605
High resolution limit [Å]	2.560	2.560
Number of reflections	547294	26960
<I/σ(I)>	8.5	1.4
Completeness [%]	100.0	100
Redundancy	12.1	12
CC(1/2)	0.987	0.342

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	293	18.7 % PEG 3350, 0.1 M CBTP, PH 7.0