Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-05-23 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.619 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 37.068, 60.827, 42.223 |
| Unit cell angles | 90.00, 112.54, 90.00 |
Refinement procedure
| Resolution | 30.432 - 2.331 |
| Rwork | 0.222 |
| R-free | 0.31370 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.444 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.432 | 2.410 |
| High resolution limit [Å] | 2.330 | 2.330 |
| Number of reflections | 7445 | 688 |
| <I/σ(I)> | 5.4 | |
| Completeness [%] | 99.6 | |
| Redundancy | 3.5 | |
| CC(1/2) | 0.955 | 0.296 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 9.6 | 291.15 | 20% (w/v) PEG-8000, 0.1M CHES pH 9.5 (final pH 9.6) |
