9Q9M
Cryo-EM structure of human Mre11-Rad50-Nbs1 (MRN) complex bound to DNA and telomeric factor TRF2
EM specimen
| Vitrification | Yes |
| Instrument | LEICA PLUNGER |
EM Data Collection
| Microscope | TFS KRIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Cs (mm) | 2.7 |
| Energy Filter | GIF Bioquantum |
| Detector | FEI FALCON IV (4k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 2.81 |
| Software | cryoSPARC,EPU,UCSF ChimeraX,PHENIX |
| CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |
