9Q83

Crystal structure of Lotus japonicus CHIP13 in complex with chitooctaose

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P13 (MX1)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-10-04
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97
Spacegroup name	C 1 2 1
Unit cell lengths	133.786, 70.751, 91.984
Unit cell angles	90.00, 117.94, 90.00

Refinement procedure

Resolution	64.200 - 2.010
R-factor	0.207
Rwork	0.205
R-free	0.24350
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.753
Data reduction software	XDS
Data scaling software	STARANISO
Phasing software	PHASER
Refinement software	PHENIX (1.21.2_5419)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	80.398	2.300
High resolution limit [Å]	2.010	2.024
Rmeas	0.256	1.312
Rpim	0.096	0.494
Number of reflections	25349	1267
<I/σ(I)>	7.3	1.7
Completeness [%]	51.6
Redundancy	7
CC(1/2)	0.990	0.597

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.5	292.15	0.15M LiSO4, 0.075 M NaAc pH 4.5 22.5% PEG-8000