9Q7Z

One of a series of engineered variants of I-OnuI meganuclease targeting altered DNA target sequence

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	108
Detector technology	PIXEL
Collection date	2017-12-05
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.99999
Spacegroup name	C 1 2 1
Unit cell lengths	327.795, 40.472, 78.235
Unit cell angles	90.00, 94.01, 90.00

Refinement procedure

Resolution	40.874 - 3.319
R-factor	0.244
Rwork	0.239
R-free	0.28590
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.003
RMSD bond angle	0.533
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER (2.7.16)
Refinement software	PHENIX ((1.13_2998: ???))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	3.420
High resolution limit [Å]	3.300	7.100	3.300
Rmerge	0.090	0.073	0.154
Rmeas	0.100	0.081	0.178
Rpim	0.042	0.033	0.087
Total number of observations	79880
Number of reflections	15108	1655	1227
<I/σ(I)>	12.2
Completeness [%]	97.0	99.4	79.8
Redundancy	5.3	5.6	3.4
CC(1/2)		0.995	0.994

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	150MM DL-Malic acid pH 7.0, 25% PEG 3350