9Q78

Crystal structure of T. cruzi EIF4E6 in complex with EIF4G5 peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-07-19
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97856
Spacegroup name	P 1 21 1
Unit cell lengths	39.893, 112.694, 100.024
Unit cell angles	90.00, 97.96, 90.00

Refinement procedure

Resolution	49.580 - 2.100
R-factor	0.20454
Rwork	0.201
R-free	0.26977
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	0.840
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0430)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	112.690	2.190
High resolution limit [Å]	2.100	2.100
Number of reflections	37674	1884
<I/σ(I)>	7.5
Completeness [%]	94.4
Redundancy	7
CC(1/2)	0.997	0.767

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	Complex at 7.3 mg/mL in 20 mM Tris-HCl, 150 mM NaCl, 2 mM DTT, pH 8.0 with crystallization buffer containing 80 mM Monosaccharides (0.2 M D-glucose, 0.2 M D-mannose, 0.2 M D-galactose, 0.2 M L-fucose, 0.2 M D-xylose, 0.2 M N-acetyl-D-glucosamine), 100 mM Buffer System 1 (pH 6.5; 0.4 M imidazole, 0.6 M MES monohydrate), and 37.5% (w/v) Precipitant Mix 4 (25% v/v MPD, 25% v/v PEG 1000, 25% w/v PEG 3350)