9Q73

Crystal structure of T. brucei EIF4E5 in complex with EIF4G1 peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-12-07
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97856
Spacegroup name	P 3 2 1
Unit cell lengths	140.410, 140.410, 41.223
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	18.810 - 1.660
R-factor	0.1382
Rwork	0.137
R-free	0.15688
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.012
RMSD bond angle	0.995
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0430)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	121.600	1.830
High resolution limit [Å]	1.660	1.660
Number of reflections	39342	1967
<I/σ(I)>	15
Completeness [%]	95.3
Redundancy	21
CC(1/2)	0.999	0.864

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	Complex at 9.1 mg/mL in 20 mM Tris-HCl, 150 mM NaCl, 2 mM DTT, pH 8.0 with crystallization solution containing 0.3 M Carboxylic Acids (0.2 M sodium formate, 0.2 M ammonium acetate, 0.2 M sodium citrate tribasic dihydrate, 0.2 M potassium sodium tartrate tetrahydrate, 0.2 M sodium oxamate), 0.12 M Alcohols (0.2 M 1,6-hexanediol, 0.2 M 1-butanol, 0.2 M 1,2-propanediol, 0.2 M 2-propanol, 0.2 M 1,4-butanediol, 0.2 M 1,3-propanediol), 0.1 M Buffer System 1 (pH 6.5; 0.4 M imidazole, 0.6 M MES monohydrate), and 35% Precipitant Mix 4 (25% v/v MPD, 25% v/v PEG 1000, 25% w/v PEG 3350)