9Q72

Trypanosoma brucei EIF4E5 translation initiation factor in complex with cap-4

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 2
Synchrotron site	SOLEIL
Beamline	PROXIMA 2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-12-12
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.98011
Spacegroup name	P 31 2 1
Unit cell lengths	72.660, 72.660, 95.260
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	37.980 - 1.250
R-factor	0.14067
Rwork	0.139
R-free	0.16890
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.012
RMSD bond angle	1.884
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0430)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	37.980	3.563
High resolution limit [Å]	1.250	1.252
Number of reflections	80904	3729
<I/σ(I)>	23.1
Completeness [%]	96.1
Redundancy	19.9
CC(1/2)	0.999	0.615

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	Protein at 7.5 mg/mL, in 20 mM Tris-HCl, 150 mM NaCl, 2 mM DTT, pH 8.0 (pre-incubated with cap-4) with crystallization solution containing 0.03 M Divalents, 0.1 M buffer system 1 (pH 6.5), 24% precipitant Mix, from Morpheus Screen