9Q5C
Cryo EM structure of alpha-glucosidase (yicI) from Klebsiella aerogenes
EM specimen
| Vitrification | Yes |
| Instrument | FEI VITROBOT MARK IV |
EM Data Collection
| Microscope | TFS KRIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Cs (mm) | 2.7 |
| Detector | GATAN K3 (6k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 3.19 |
| Number of Particle | 925341 |
| Software | Appion,cryoSPARC,Leginon,UCSF ChimeraX,PHENIX |
| Symmetry | D3 |
| CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |
