9Q4F

Measles Virus Fusion Glycoprotein Postfusion Core (Wild-type Variant)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-1
Synchrotron site	NSLS-II
Beamline	17-ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2025-05-25
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.91969
Spacegroup name	P 1 21 1
Unit cell lengths	49.551, 53.984, 70.960
Unit cell angles	90.00, 92.72, 90.00

Refinement procedure

Resolution	32.910 - 1.950
R-factor	0.2001
Rwork	0.195
R-free	0.25090
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.041
RMSD bond angle	0.565
Data reduction software	XDS (Jun 30, 2024 (BUILT 20241002))
Data scaling software	Aimless (0.7.9)
Phasing software	PHASER
Refinement software	PHENIX (1.21.2_5419)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	32.910	2.110
High resolution limit [Å]	1.950	1.950
Rmerge	0.150	1.120
Rmeas	0.170
Rpim	0.080	0.600
Number of reflections	21439	1072
<I/σ(I)>	5.9
Completeness [%]	91.6	54.2
Redundancy	4.4
CC(1/2)	0.926	0.309

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	281	30mM Sodium fluoride; 30mM Sodium bromide; 30mM Sodium iodide, 100mM Imidazole/MES monohydrate (acid), 12.5% (v/v) MPD; 12.5% (v/v) PEG 1000; 12.5% (w/v) PEG 3350 (pH 6.5)