9Q3O

Crystal structure of human CDK5-cyclinB1 complex bound to AMP-PNP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-02-18
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.979460
Spacegroup name	P 32 2 1
Unit cell lengths	120.859, 120.859, 116.070
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	39.560 - 2.500
R-factor	0.1972
Rwork	0.195
R-free	0.24880
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.002
RMSD bond angle	0.528
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((1.21_5207: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.560	2.600
High resolution limit [Å]	2.500	2.500
Number of reflections	34310	3800
<I/σ(I)>	9.4
Completeness [%]	99.9	99.6
Redundancy	10.4	10.1
CC(1/2)	0.998	0.287

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	10% PEG 3350 200 mM Imidazole/Malate pH 6.0 5% Mg Formate (saturated) 2.3 mM AMP-PNP was added to the protein