9Q3D

[20-7B C|A] 20 bp tensegrity triangle that propagates via blunt-end stacking with C stacking on A at the interface

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 17-ID
Synchrotron site	APS
Beamline	17-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-12-11
Detector	DECTRIS EIGER X 9M
Wavelength(s)	1.007430
Spacegroup name	P 63
Unit cell lengths	125.390, 125.390, 55.340
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	54.300 - 5.030
R-factor	0.281
Rwork	0.281
R-free	0.28370
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.007
Data reduction software	autoPROC
Data scaling software	STARANISO
Phasing software	PHASER
Refinement software	PHENIX (1.21.2_5419)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	62.695	62.695	5.047
High resolution limit [Å]	5.030	22.963	5.030
Number of reflections	2202	24	23
<I/σ(I)>	10.9
Completeness [%]	99.8
Redundancy	19.2
CC(1/2)	1.000	1.000	0.046

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	100 mM MOPS, 1.25 M magnesium sulfate