9Q1H

A7 minibinder in complex with Abp2D

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 4.2.2
Synchrotron site	ALS
Beamline	4.2.2
Temperature [K]	100
Detector technology	CMOS
Collection date	2024-06-24
Detector	RDI CMOS_8M
Wavelength(s)	1.00003
Spacegroup name	P 21 21 21
Unit cell lengths	25.806, 52.464, 152.121
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.600 - 1.350
Rwork	0.165
R-free	0.19710
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	0.963
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX (1.20.1_4487)
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.600	1.398
High resolution limit [Å]	1.350	1.350
Rmerge	0.021
Number of reflections	46366	4424
<I/σ(I)>	17.87	2.02
Completeness [%]	99.4	97.34
Redundancy	2	2
CC(1/2)	0.999	0.759

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293.15	0.1M Sodium acetate pH 4.6, 0.15 M Ammonium acetate, and 25% PEG 4000