Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 31-ID |
| Synchrotron site | APS |
| Beamline | 31-ID |
| Temperature [K] | 88 |
| Detector technology | PIXEL |
| Collection date | 2019-05-09 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.979310 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 243.416, 47.866, 180.655 |
| Unit cell angles | 90.00, 129.18, 90.00 |
Refinement procedure
| Resolution | 38.090 - 3.703 |
| R-factor | 0.2188 |
| Rwork | 0.216 |
| R-free | 0.25270 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.700 |
| Data reduction software | XDS |
| Data scaling software | STARANISO |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.11.8 (10-JUL-2024)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 38.090 | 3.790 |
| High resolution limit [Å] | 3.703 | 3.703 |
| Number of reflections | 13751 | 689 |
| <I/σ(I)> | 7.4 | 2.3 |
| Completeness [%] | 77.4 | |
| Redundancy | 3.3 | |
| CC(1/2) | 0.991 | 0.874 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 100mM Sodium Cacodylate pH 5.5, 15% w/v PEG 6k, 0.15M Potassium Thiocyanate |
