9Q1F

Choanoflagellate Salpingoeca macrocollata STING

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-1
Synchrotron site	NSLS-II
Beamline	17-ID-1
Temperature [K]	80
Detector technology	PIXEL
Collection date	2023-10-08
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.92010
Spacegroup name	P 1 21 1
Unit cell lengths	66.946, 88.743, 76.023
Unit cell angles	90.00, 94.14, 90.00

Refinement procedure

Resolution	34.040 - 2.650
R-factor	0.2457
Rwork	0.243
R-free	0.27480
Structure solution method	SAD
RMSD bond length	0.003
RMSD bond angle	0.622
Data reduction software	autoPROC
Data scaling software	autoPROC
Phasing software	PHENIX
Refinement software	PHENIX (1.21.1_5286)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	34.040	2.710
High resolution limit [Å]	2.650	2.650
Number of reflections	25932	1855
<I/σ(I)>	4.51
Completeness [%]	99.7
Redundancy	7.1
CC(1/2)	0.984	0.378

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.65	293	5% ethylene glycol, 100 mM MOPS pH 6.65, and 10% (w/v) PEG8000