9Q0P

Human anti-EBV gH/gL Fab (AMMO1) with G111T light chain mutation

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-12-12
Detector	DECTRIS EIGER2 XE 16M
Wavelength(s)	0.97946
Spacegroup name	P 21 21 21
Unit cell lengths	50.722, 69.774, 124.659
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.340 - 2.080
R-factor	0.1933
Rwork	0.189
R-free	0.24270
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	0.850
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.20.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.340	2.157
High resolution limit [Å]	2.080	2.083
Number of reflections	26825	2491
<I/σ(I)>	15.22
Completeness [%]	98.5
Redundancy	2
CC(1/2)	0.998	0.874

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	170 mM ammonium acetate, 85 mM sodium citrate-HCl, pH 5.6, 25.5% (w/v) PEG 4000, and 15% (v/v) glycerol