9Q0J
Structure of dsRNA 16mer containing 4'-C-methyluridine
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-1 |
| Synchrotron site | SSRL |
| Beamline | BL12-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-07-05 |
| Detector | DECTRIS EIGER2 XE 16M |
| Wavelength(s) | 0.92002 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 74.081, 42.928, 49.294 |
| Unit cell angles | 90.00, 120.02, 90.00 |
Refinement procedure
| Resolution | 32.143 - 2.000 |
| Rwork | 0.225 |
| R-free | 0.24990 |
| Structure solution method | SAD |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.691 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | AutoSol |
| Refinement software | REFMAC (5.8.0430) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 33.000 | 2.050 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.075 | 0.622 |
| Rmeas | 0.090 | 0.741 |
| Rpim | 0.049 | 0.398 |
| Number of reflections | 9056 | 687 |
| <I/σ(I)> | 5.3 | |
| Completeness [%] | 98.5 | 98.8 |
| Redundancy | 3.3 | 3.4 |
| CC(1/2) | 0.994 | 0.691 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 294 | 10% MPD, 40 mM Sodium cacodylate trihydrate pH 6.0 12 mM Spermine, 80 mM KCl |
