9Q0I
Structure of dsRNA 16mer containing 4'-C-methyladenosine
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-1 |
| Synchrotron site | SSRL |
| Beamline | BL12-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-07-05 |
| Detector | DECTRIS EIGER2 XE 16M |
| Wavelength(s) | 0.91943 |
| Spacegroup name | P 31 |
| Unit cell lengths | 43.038, 43.038, 127.318 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 37.300 - 1.801 |
| Rwork | 0.212 |
| R-free | 0.24590 |
| Structure solution method | SAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.803 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | AutoSol |
| Refinement software | REFMAC (5.8.0430) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.300 | 1.840 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.090 | 1.225 |
| Rmeas | 0.094 | 1.320 |
| Rpim | 0.029 | 0.482 |
| Number of reflections | 24409 | 1436 |
| <I/σ(I)> | 11.5 | 1.6 |
| Completeness [%] | 99.7 | 98.7 |
| Redundancy | 10.3 | 7.3 |
| CC(1/2) | 0.999 | 0.264 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 294 | 10% MPD, 40 mM Sodium cacodylate trihydrate pH 6.0 12 mM Spermine, 80 mM KCl |
