9Q0B

CTX-M-15 WT in complex with BLIP E73W

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.1
Synchrotron site	ALS
Beamline	8.2.1
Temperature [K]	298
Detector technology	CCD
Collection date	2022-09-02
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.1
Spacegroup name	I 1 2 1
Unit cell lengths	47.460, 81.960, 129.074
Unit cell angles	90.00, 96.14, 90.00

Refinement procedure

Resolution	40.980 - 1.580
R-factor	0.164
Rwork	0.163
R-free	0.18160
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	0.824
Data reduction software	iMOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX (1.21.2_5419)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.980	1.600
High resolution limit [Å]	1.580	1.580
Rmerge	0.059
Number of reflections	65474	2804
<I/σ(I)>	15.43
Completeness [%]	96.9	97.97
Redundancy	4.8
CC(1/2)	0.991	0.977

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	0.1 M MES pH 6.5, 25% (w/v) PEG 4000