9PZH

One of a series of engineered variants of I-OnuI meganuclease targeting altered DNA target sequence

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.1
Synchrotron site	ALS
Beamline	5.0.1
Temperature [K]	108
Detector technology	PIXEL
Collection date	2017-06-15
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.977408
Spacegroup name	P 21 21 21
Unit cell lengths	39.312, 76.317, 168.321
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.664 - 2.356
R-factor	0.1836
Rwork	0.178
R-free	0.23340
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.012
RMSD bond angle	1.266
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER (2.7.16)
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.450
High resolution limit [Å]	2.356	5.100	2.370
Rmerge	0.097	0.071	0.306
Rmeas	0.107	0.079	0.344
Rpim	0.044	0.034	0.154
Number of reflections	21544	2332	2019
<I/σ(I)>	10.2
Completeness [%]	99.1	98.7	93.9
Redundancy	5.8	5.2	4.7
CC(1/2)		0.992	0.928

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	100 mM HEPES, pH 6.5, 200 mM ammonium sulfate, 25% PEG3350