9PZ7

One of a series of engineered variants of I-OnuI meganuclease targeting altered DNA target sequence

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	108
Detector technology	CCD
Collection date	2018-02-02
Detector	RIGAKU SATURN 944+
Wavelength(s)	1.54
Spacegroup name	P 21 21 21
Unit cell lengths	49.208, 63.739, 166.752
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	31.898 - 2.504
R-factor	0.2213
Rwork	0.218
R-free	0.24780
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER (2.8.1)
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.590
High resolution limit [Å]	2.500	5.380	2.500
Rmerge	0.132	0.057	0.713
Rmeas	0.138	0.060	0.762
Rpim	0.040	0.018	0.261
Total number of observations	212630
Number of reflections	18703	2024	1810
<I/σ(I)>	6.8
Completeness [%]	99.5	99.4	97.5
Redundancy	11.4	11.3	7.7
CC(1/2)		0.998	0.797

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	100 mM HEPES, pH 7.5, 200 mM ammonium sulfate, 25% PEG3350