9PZ6

Anti-NANP Fab with G112T light chain mutation

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-06-03
Detector	DECTRIS EIGER2 XE 16M
Wavelength(s)	0.97946
Spacegroup name	P 21 21 21
Unit cell lengths	46.974, 71.761, 137.364
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.300 - 1.700
R-factor	0.1988
Rwork	0.197
R-free	0.23070
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.975
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.19.1_4122)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.300	1.761
High resolution limit [Å]	1.700	1.700
Number of reflections	51978	3934
<I/σ(I)>	6.66
Completeness [%]	96.9
Redundancy	13.1
CC(1/2)	0.994	0.177

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	200 mM ammonium acetate, 100 mM Bis-Tris-HCl, pH 5.5, 25% (w/v) PEG 3350