9PYC
Cryo-EM structure of EF-G and RaiA simultaneously bound to an E. coli ribosome imaged in a cell lysate
EM specimen
| Vitrification | Yes |
| Instrument | FEI VITROBOT MARK IV |
EM Data Collection
| Microscope | TFS KRIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Detector | GATAN K3 BIOQUANTUM (6k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 2.9 |
| Number of Particle | 1503396 |
| Software | Warp,cryoSPARC,cryoDRGN,PHENIX |
| CTF correction | NONE |
