9PY2
Engaged-state loperamide-mu opioid receptor-Gi GDPbS complex (rebound)
This is a non-PDB format compatible entry.
EM specimen
| Vitrification | Yes |
| Instrument | FEI VITROBOT MARK IV |
EM Data Collection
| Microscope | TFS KRIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Energy Filter | GIF Bioquantum |
| Detector | GATAN K3 BIOQUANTUM (6k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 3.16 |
| Software | cryoSPARC,EPU |
| CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |
