9PY0

PRMT5 Bound to Compound 12 and MTA

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 17-ID
Synchrotron site	APS
Beamline	17-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-02-16
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.00000
Spacegroup name	I 2 2 2
Unit cell lengths	103.420, 137.669, 178.065
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.340 - 2.360
R-factor	0.2328
Rwork	0.230
R-free	0.28300
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.659
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.21.2_5419)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	41.340	2.400
High resolution limit [Å]	2.360	2.360
Rmerge	0.096	1.509
Rmeas	0.104	1.638
Rpim	0.040	0.632
Number of reflections	52658	2599
<I/σ(I)>	14.75	0.99
Completeness [%]	99.7
Redundancy	6.9
CC(1/2)	0.998	0.749

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277.15	11-22% PEG 3350, 200 mM ammonium sulfate