9PW8

Fem-1 homolog B (FEM1B) in complex with VU0417412

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.1
Synchrotron site	ALS
Beamline	8.2.1
Temperature [K]	293
Detector technology	CMOS
Collection date	2024-12-13
Detector	RDI CMOS_8M
Wavelength(s)	1.0
Spacegroup name	P 4 21 2
Unit cell lengths	123.708, 123.708, 137.786
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.030 - 2.800
R-factor	0.3014
Rwork	0.299
R-free	0.33780
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	1.214
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.21.2_5419)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.030	2.860
High resolution limit [Å]	2.730	2.730
Number of reflections	755781	93515
<I/σ(I)>	11.6
Completeness [%]	100.0
Redundancy	26
CC(1/2)	0.998	0.368

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	298	0.1 M HEPES pH 7.5, 0.25% PEG 8000, 1.2-1.6 M Ammonium sulfate, 0.05-0.1 M Sodium thiocyanate