9PVX
RNA polymerase II elongation complex with dA at +1 site, 8-oxo-GTP bound in E-site.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL12-2 |
| Synchrotron site | SSRL |
| Beamline | BL12-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2024-03-07 |
| Detector | DECTRIS EIGER2 XE 16M |
| Wavelength(s) | 0.97946 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 159.703, 222.316, 192.114 |
| Unit cell angles | 90.00, 97.92, 90.00 |
Refinement procedure
| Resolution | 38.500 - 3.390 |
| R-factor | 0.193 |
| Rwork | 0.192 |
| R-free | 0.23570 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.640 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.21.2_5419: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.820 | 3.450 |
| High resolution limit [Å] | 3.390 | 3.390 |
| Rmerge | 0.253 | 2.030 |
| Rmeas | 0.273 | 2.181 |
| Rpim | 0.101 | 0.795 |
| Total number of observations | 659344 | 33711 |
| Number of reflections | 91036 | 4539 |
| <I/σ(I)> | 6.5 | 1.4 |
| Completeness [%] | 99.2 | |
| Redundancy | 7.2 | 7.4 |
| CC(1/2) | 0.980 | 0.591 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | 390mM (NH4)2HPO4/NaH2PO4, pH 6.5, 5mM dioxane, 5mM DTT, 10-12% PEG6000 |
