9PVU
RNA polymerase II elongation complex with dC at +1 site, 8-oxo-GMP added.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.1 |
| Synchrotron site | ALS |
| Beamline | 5.0.1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2023-11-06 |
| Detector | DECTRIS PILATUS3 2M |
| Wavelength(s) | 0.97741 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 165.999, 223.510, 192.778 |
| Unit cell angles | 90.00, 100.26, 90.00 |
Refinement procedure
| Resolution | 49.320 - 3.310 |
| R-factor | 0.2441 |
| Rwork | 0.243 |
| R-free | 0.28130 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.605 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.21.2_5419: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.320 | 3.370 |
| High resolution limit [Å] | 3.310 | 3.310 |
| Rmerge | 0.481 | |
| Number of reflections | 102682 | 5031 |
| <I/σ(I)> | 5.2 | 0.5 |
| Completeness [%] | 100.0 | |
| Redundancy | 7 | |
| CC(1/2) | 0.981 | 0.231 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | 390mM (NH4)2HPO4/NaH2PO4, pH 6.5, 5mM dioxane, 5mM DTT, 10-12% PEG6000 |
