9PUZ

HRAS complex with UM0153030 compound

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08B1-1
Synchrotron site	CLSI
Beamline	08B1-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-03-17
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97918
Spacegroup name	P 63
Unit cell lengths	85.720, 85.720, 136.270
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	37.120 - 2.000
R-factor	0.2117
Rwork	0.209
R-free	0.25340
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.784
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	37.120	2.071
High resolution limit [Å]	2.000	2.000
Rmerge	0.058	1.001
Rmeas	0.061	1.056
Rpim	0.019	0.335
Number of reflections	38269	3713
<I/σ(I)>	20.79	2.65
Completeness [%]	99.6	99.9
Redundancy	10.1	9.9
CC(1/2)	0.999	0.861

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.7	293.15	0.1 M Tris, 0.2 M CaCl2, 22 % PEG 4,000