Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-2 |
| Synchrotron site | SSRL |
| Beamline | BL9-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-10-29 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9600 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 46.688, 94.415, 105.140 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 41.886 - 0.944 |
| Rwork | 0.126 |
| R-free | 0.14660 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.028 |
| RMSD bond angle | 2.473 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | REFMAC |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 41.886 | 0.960 |
| High resolution limit [Å] | 0.940 | 0.940 |
| Rpim | 0.009 | 0.798 |
| Number of reflections | 139644 | 5222 |
| <I/σ(I)> | 31.4 | 0.8 |
| Completeness [%] | 93.7 | 71.6 |
| Redundancy | 35.1 | 20.4 |
| CC(1/2) | 1.000 | 0.449 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.5 | 294 | 1 micro-liter protein containing 3 mM Biotin in 10 mM Tris-HCl pH 7.0 mixed with 1 micro-liter 38% Ammonium Sulfate, 100 mM Na-Acetate pH 4.5, 200 mM NaCl at 21C (294 K) |
