9PU3

HRAS complex with UM0140692 compound

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 4.2.2
Synchrotron site	ALS
Beamline	4.2.2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-02-23
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97918
Spacegroup name	C 1 2 1
Unit cell lengths	63.754, 37.232, 63.070
Unit cell angles	90.00, 91.22, 90.00

Refinement procedure

Resolution	63.060 - 1.700
R-factor	0.1754
Rwork	0.171
R-free	0.21690
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	0.964
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	63.060	1.761
High resolution limit [Å]	1.700	1.700
Rmerge	0.065	0.151
Rmeas	0.079	0.183
Number of reflections	15867	1502
<I/σ(I)>	15.96	6.63
Completeness [%]	96.1	91.7
Redundancy	3.2	6.63
CC(1/2)	0.995	0.978

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	Tris