9PTG

Crystal structure of C. elegans PUF-3 in complex with pos-1 RNA mut2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-1
Synchrotron site	NSLS-II
Beamline	17-ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2023-07-15
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.0
Spacegroup name	P 32 2 1
Unit cell lengths	161.825, 161.825, 99.734
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	42.300 - 2.800
R-factor	0.2409
Rwork	0.239
R-free	0.27200
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.095
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.21.2_5419)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.790	2.900
High resolution limit [Å]	2.800	2.800
Rmerge	0.126	1.622
Rmeas	0.136
Rpim	0.051	0.640
Number of reflections	36648	3640
<I/σ(I)>	7.9	1.5
Completeness [%]	98.1
Redundancy	6.95
CC(1/2)	0.998	0.877

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	3.0 ~ 3.5 M sodium formate, 0.1 M bis-tris propane pH 5.0 ~ 6.0