9PTF

Stabilized Y188N variant of the internal UBA Domain of HHR23A in the P43 space group

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2024-07-16
Detector	DECTRIS EIGER2 XE 16M
Wavelength(s)	0.97946
Spacegroup name	P 43
Unit cell lengths	31.240, 31.240, 40.308
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	24.690 - 1.200
R-factor	0.1931
Rwork	0.192
R-free	0.21530
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.005
RMSD bond angle	0.633
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.21.2_5419)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	24.690	1.241
High resolution limit [Å]	1.198	1.198
Rmerge	0.072	0.599
Rmeas	0.079	0.664
Rpim	0.031	0.280
Number of reflections	60759	4947
<I/σ(I)>	10.37	2.07
Completeness [%]	90.9
Redundancy	5.8	5.2
CC(1/2)	0.997	0.920

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293.15	0.2 M magnesium acetate tetrahydrate, 0.1 M sodium cacodylate (pH 6.5), 20% w/v PEG 8000