9PTD

Crystal structure of C. elegans PUF-3

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-04-02
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.0
Spacegroup name	P 43
Unit cell lengths	251.552, 251.552, 39.432
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.470 - 3.140
R-factor	0.2423
Rwork	0.241
R-free	0.27510
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.704
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.21.2_5419)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	3.200
High resolution limit [Å]	3.140	8.540	3.150
Rmerge	0.151	0.076	0.940
Rmeas	0.169	0.086	1.041
Rpim	0.074	0.038	0.443
Number of reflections	44271	2434	2183
<I/σ(I)>	6.6
Completeness [%]	99.6	99.9	100
Redundancy	5.1	4.7	5.4
CC(1/2)	0.979	0.988	0.630

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		296	0.2 M sodium malonate, pH 6.5, 20% (w/v) PGE 3350