9PT1

Q108K:K40L:T51V:T53S:R58W:Y19W:L117E mutant of hCRBPII bound to fluorophore TD-1V-10

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-D
Synchrotron site	APS
Beamline	21-ID-D
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-12-19
Detector	DECTRIS EIGER X 9M
Wavelength(s)	1.0
Spacegroup name	C 1 2 1
Unit cell lengths	29.396, 66.751, 64.067
Unit cell angles	90.00, 90.40, 90.00

Refinement procedure

Resolution	33.380 - 1.480
Rwork	0.175
R-free	0.19470
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	0.934
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.212-5419)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	33.380	1.530
High resolution limit [Å]	1.480	1.480
Rmerge	0.041	0.482
Number of reflections	19317	1927
<I/σ(I)>	41.29
Completeness [%]	93.4
Redundancy	6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	PEG4000, ammonium acetate, 100 mM sodium acetate, pH 4.0 - 4.8