9PSX
Q108K:K40L:T51V:T53S:R58W:Y19W:L117E mutant of hCRBPII bound to fluorophore TD-1V-3
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-D |
| Synchrotron site | APS |
| Beamline | 21-ID-D |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-12-19 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 1.127 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 29.541, 66.450, 63.861 |
| Unit cell angles | 90.00, 90.38, 90.00 |
Refinement procedure
| Resolution | 29.470 - 1.290 |
| R-factor | 0.1751 |
| Rwork | 0.174 |
| R-free | 0.18640 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.189 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.21.2_5419) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.470 | 1.330 |
| High resolution limit [Å] | 1.280 | 1.280 |
| Rmerge | 0.080 | 0.534 |
| Number of reflections | 30407 | 2898 |
| <I/σ(I)> | 17.2 | |
| Completeness [%] | 96.9 | |
| Redundancy | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | PEG4000, ammonium acetate, 100 mM sodium acetate, pH 4.0 - 4.8 |
