9PSE

In situ MicroED structure of IL-5 activated human eosinophil major basic protein-1

Experimental procedure

Spacegroup name	P 1 21 1
Unit cell lengths	30.200, 57.800, 58.600
Unit cell angles	90.00, 91.20, 90.00

Refinement procedure

Resolution	3.180 - 3.180
R-factor	0.2815
Rwork	0.278
R-free	0.31950
RMSD bond length	0.002
RMSD bond angle	0.424
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX
Refinement software	PHENIX (1.21.1_5286)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	30.690	30.690	3.440
High resolution limit [Å]	3.000	4.330	3.000
Rmerge	0.660	0.552	1.376
Rmeas	0.724	0.604	1.585
Total number of observations	35354
Number of reflections	6805	2581	1878
<I/σ(I)>	2.47	3.47	1.16
Completeness [%]	85.7
CC(1/2)	0.682	0.663	0.275