9PSB

Crystal structure of C. elegans PUF-3 in complex with pos-1 RNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-10-07
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.0
Spacegroup name	P 32 2 1
Unit cell lengths	160.298, 160.298, 99.531
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	42.280 - 3.250
R-factor	0.2476
Rwork	0.246
R-free	0.28010
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.104
Data reduction software	XDS
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.21.2_5419)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	3.310
High resolution limit [Å]	3.250	8.810	3.250
Rmerge	0.176	0.068	1.017
Rmeas	0.181	0.070	1.048
Rpim	0.041	0.016	0.251
Total number of observations	433939
Number of reflections	23087	1256	1130
<I/σ(I)>	3.4
Completeness [%]	98.3	99.1	99.1
Redundancy	18.8	18.3	17.1
CC(1/2)	0.995	0.999	0.966

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		296	3.0 ~ 3.5 M sodium formate, 0.1 M bis-tris propane pH 5.0 ~ 6.0