9PR8
Crystal Structure of the Clostridiodes difficile CspC-CspA heterodimer.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-12-09 |
| Detector | DECTRIS EIGER2 X 16M |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 71.143, 105.505, 311.325 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 99.920 - 3.350 |
| R-factor | 0.2478 |
| Rwork | 0.246 |
| R-free | 0.27820 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.353 |
| Data reduction software | RAPD |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.21.2_5419) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 99.920 | 3.450 |
| High resolution limit [Å] | 3.350 | 3.350 |
| Number of reflections | 34565 | 2745 |
| <I/σ(I)> | 3.6 | |
| Completeness [%] | 99.7 | 97.65 |
| Redundancy | 12.3 | |
| CC(1/2) | 0.970 | 0.370 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 300 | 0.2M magnesium chloride, 0.1M Tris-HCl pH 8.5, and 20% w/v PEG 8000 |
