9PNT
N4 vRNAP gp50 - open complex
EM specimen
| Vitrification | Yes |
EM Data Collection
| Microscope | TFS KRIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Detector | TFS FALCON 4i (4k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 4.3 |
| Software | cryoSPARC,PHENIX,Coot,ISOLDE |
| CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |
